12/16/2023 0 Comments Pulsar lost colony infectionaeruginosa is present at low proportions in the community and yet, it plays a critical role in community function, suggesting it acts as a keystone species in this community. This biofilm community displays higher biomass and increased resilience to antimicrobial stress conditions such as sodium dodecyl sulfate and tobramycin, compared to monospecies biofilm populations. Here, we have used a model, mixed-species biofilm community comprised of Pseudomonas aeruginosa PAO1, Pseudomonas protegens Pf-5 and Klebsiella pneumoniae KP1. aeruginosa 2, lasRrhlR 3, lasRrhlR/pUCP22notI+R2.īacterial biofilms are important medically, environmentally and industrially and there is a need to understand the processes that govern functional synergy and dynamics of species within biofilm communities. **** denotes significant difference by One-way ANOVA followed by Tukey’s post-test ( P<0.0001). The data are averages from three independent biological replicates. Bars represent OD 495 values obtained after subtracting the values of medium control from the sample values. Elastase activity was calculated by measuring the absorbance (OD 495) of the supernatant. (C) Elastase activity of indicated strains measured by mixing 100 µl of culture supernatant with 900 µl of Elastin-Congo Red containing buffer and incubating overnight at 37☌. Bars represent average diameter of the zone of clearance around the colonies. (B) Protease activity of indicated strains on 1% skimmed milk containing LB agar plates. Bars represent average diameter of the swarm area. (A) Swarming motility of indicated strains on 0.5% swarm agar. ![]() ![]() aeruginosa wild type, lasRrhlR mutant and the complemented strain. Supplementary Figure 1: QS regulated phenotypes of P.
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